黑色丝袜女神呻吟小道具在线­_欧美粉嫩被两个粗黑疯狂进出­_日产中文字乱码卡一卡二卡­_一本色道在线久88在线观看片­_差差答答网页人口

技術(shù)文章您的位置:網(wǎng)站首頁 >技術(shù)文章 > 化學腫瘤基因組學實驗室使用英克胎牛血清發(fā)表已見刊
化學腫瘤基因組學實驗室使用英克胎牛血清發(fā)表已見刊
更新時間:2022-05-24   點擊次數(shù):662次

清華大學化學腫瘤基因組學國家重點實驗室使用Invigentech胎牛血清發(fā)表文章到Cell Reports Medicine已見刊。


一、2020第四季度,清華大學化學腫瘤基因組學國家重點實驗室發(fā)表標題為:An Automated Organoid Platform with Inter-organoid Homogeneity and Inter-patient Heterogeneity的文章到Cell Reports Medicine;

二、使用invigentech(英克)A6903FBS-500,FBS Uruguay Origin胎牛血清對小鼠肺,肝,腎,胰腺,脾臟, 心臟等細胞進行培養(yǎng);

三、文章部分內(nèi)容見下:

Mouse tissue processing

BALB/c mice (male, 6 - 8-weeks old) were euthanized by excessive CO2. Then, the organs (lung, liver, kidney, pancreas, spleen,heart) were isolated and cut into small pieces (1 - 3 mm3 for each piece). After being washed with cold PBS (1 3 ) for three times and supplemented with 2.0% penicillin-streptomycin (vol/vol, GIBCO), the tissues were digested by 1.0 mg/mL collagenase type I (Sigma-Aldrich) and 2.0% fetal bovine serum (FBS) (vol/vol, Invigentech) on an orbital shaker at 37C and incubated for 1 - 2 h. After digestion, the tissues were sheared by 10 mL plastic pipettes. The suspension was passed through a 100 mm filter (Falcon), and centrifuged at 1000 rpm for 5 min. The pellet was resuspended in 5 mL red blood cell lysis buffer (Roche) for 5 min at room temperature. Afterward, the suspension was recentrifuged at 1000 rpm for 5 min, and the pellet was resuspended in DMEM/F12 medium.The cell number was counted for organoid fabrication.



©2024  上海創(chuàng)凌生物科技有限公司 All Rights Reserved.  網(wǎng)站地圖  滬ICP備2023009255號-1  管理登陸  技術(shù)支持:化工儀器網(wǎng)
在線客服 二維碼

掃一掃,關(guān)注我們

阿拉尔市| 昌吉市| 神木县| 舞阳县| 广水市| 罗田县| 定安县| 临颍县| 科技| 岢岚县| 永仁县| 赤壁市| 垣曲县| 平陆县| 临朐县| 汶川县| 凌源市| 新津县| 垫江县| 昆明市| 陇南市| 手游| 都匀市| 平江县| 会昌县| 克山县| 广灵县| 三江| 扶绥县| 通江县| 江陵县| 富顺县| 汕头市| 塘沽区| 岐山县| 当阳市| 保康县| 泽州县| 华阴市| 应城市| 磴口县|